Leptin Receptor antibody

• Catalog Number: orb19243
• Category: Antibodies
• Description: Goat polyclonal antibody to Leptin Receptor
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:16000, WB: 0.1-1 μg/ml, IHC-P: 4 μg/ml
• Conjugation: Unconjugated
• MW: 132; 102; 109
• Target: Leptin Receptor
• Entrez: 3953
• Protein Sequence: TQDDIEKHQSDAG
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CD 295 antibody, anti CD295 antibody, anti LE
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 150kDa band observed in Human Brain lysates (calculated MW of 132kDa according to NP_002294.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19243 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line).

orb19243 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb19243 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb19243 (1.75µg/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Cerebral Cortex lysate using Leptin Receptor antibody

Western blot analysis of Mouse (Lane 1) and Rat (Lane 2) Brain lysate using Leptin Receptor antibody

Immunohistochemical staining of Human Heart using Leptin Receptor antibody

Immunohistochemical staining of Human Liver using Leptin Receptor antibody