JAK2 antibody

• Catalog Number: orb318917
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JAK2 also known as JAK-2 whose expression can be detected in whole-body tissues ubiquitously. It is found in the extrinsic component of the nucleus. It can perform various functions like MAPK cascade mechanism and histone H3-Y41 phosphorylation. In the nucleus it plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes the exclusion of CBX5 (HP1 alpha) from chromatin. In the cytoplasm, it plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JAK2. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: JAK2
• Entrez: 24514, 16452, 3717
• UniProt ID: Q62120, Q62689, O60674
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-Tyrosine-protein kinase JAK2 antibody, anti-J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Aguilera-Rojas, Matias et al. Deregulation of miR-27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line FEBS Open Bio, 10, 802-816 (2020)

PubMed: 32133790

Immunofluorescense analysis of Jurkat cells using JAK2 antibody

Western blot analysis of JAK2 expression in Jurkat (Lane 1), K562 (Lane 2), HEK293T (Lane 3) whole cell lysates using JAK2 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using JAK2 antibody