Lamin A antibody

• Catalog Number: orb98360
• Category: Antibodies
• Description: Mouse monoclonal to Lamin A
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 133A2
• Tested applications: FC, ICC, IHC-Fr, IHC-P, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Isotype: IgG3
• Form/Appearance: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. Since this IgG3 antibody has to be purified from the culture medium by ion-exchange chromatography it will contain remnants of bovine serum albumin.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P02545
• Source: 133A2 is a mouse monoclonal IgG3/κ antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.
• Storage: Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product
• Buffer/Preservatives: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. Since this IgG3 antibody has to be purified from the culture medium by ion-exchange chromatography it will contain remnants of bovine serum albumin.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemical staining of fiboblasts showing nuclear lamina

Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells

Immunohistochemistry on paraffin section of human colon