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Catalog Number | orb1258688 |
---|---|
Category | Antibodies |
Description | LAD1 Antibody |
Target | LAD1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 40-200 of human LAD1 (NP_005549.2). |
UniProt ID | O00515 |
MW | Observed: 54kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Ladinin-1, Lad-1, Linear IgA disease antigen, LADA Read more... |
Note | For research use only |
Western blot analysis of extracts of HeLa cells, using LAD1 antibody (orb1258688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
FA, FC, IHC-Fr, IHC-P, IP, WB | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |