L selectin antibody

• Catalog Number: orb1294260
• Category: Antibodies
• Description: Rabbit polyclonal antibody to L selectin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human L selectin / CD62L
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Conjugation: Unconjugated
• Target: L-selectin | CD62L
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti L-selectin/CD62L antibody at 1/500 dilution. Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294260 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling L-selectin/CD62L with orb1294260 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling L-selectin/CD62L with orb1294260 at 1/100.

Anti L-selectin/CD62L antibody at 1/500 dilution. Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294260 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294260 Anti-L-selectin/CD62 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.