CD44 Antibody

• Catalog Number: orb1519905
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 700 and 742 (C-term)
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD44
• UniProt ID: P16070
• NCBI: NP_000601.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: MDU2; hyaluronate receptor; IN; Indian blood group
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, 786-O, HEK293T, A-549, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse CD44 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD44 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of mouse CD44 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human CD44 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used for IP at 20 µl/mg lysate.

Detection of human CD44 in FFPE 786-O cells by ICC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.