CD44 Antibody

• Catalog Number: orb1524020
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 692 and 742
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD44
• UniProt ID: P16070
• NCBI: NP_000601.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: MDU2; hyaluronate receptor; IN; Indian blood group
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from A-549, HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524020 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of mouse CD44 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524020 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human head and neck squamous cell carcinoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524020) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB

Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human melanoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524020) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB

Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD44 antibody orb1524020 used for IP at 2 µg per reaction.