Ku80 Antibody / XRCC5

• Catalog Number: orb2637183
• Category: Antibodies
• Description: The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Ku has several functions, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity; serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: XRCC5/7315
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 300-500 was used as the immunogen for the XRCC5/Ku80 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This XRCC5/Ku80 antibody is available for research use only.
• UniProt ID: P13010
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the XRCC5/Ku80 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) MOLT-4 and 2) HEK293 cell lysate using XRCC5/Ku80 antibody (clone XRCC5/7315). Predicted molecular weight: 80-86 kDa.

IHC staining of FFPE human lymph node tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human salivary gland with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.