KRT8 Antibody

• Catalog Number: orb1252739
• Category: Antibodies
• Description: KRT8 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: K8/383
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Rat, Zebrafish
• Isotype: IgG1
• Immunogen: Recombinant human Cytokeratin 8 protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT8
• UniProt ID: P05787
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8
• Note: For research use only
• Application notes: WB: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human colon carcinoma stained with Cytokeratin 8 antibody (K8/383).

Western blot analysis of Cytokeratin 8 antibody and A431 lysate.