Cytokeratin 8 KRT8 Antibody (monoclonal, 3G9)

• Catalog Number: orb527059
• Category: Antibodies
• Description: Cytokeratin 8 KRT8 Antibody (monoclonal, 3G9)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G9
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54 kDa
• UniProt ID: P05787
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Keratin, type II cytoskeletal 8; Cytokeratin-8; CK
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody.Lane 1:human placenta tissue;2:human Caco-2 cell;3:human A549 cell;4:human A431 cell;5:human HeLa cell;6:human K562 cell;7:human HEK293 cell.

IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human intestinal cancer tissue.

IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human mammary cancer tissue.

IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in immunocytochemical section of U20S cell.

IHC analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human rectal cancer tissue.