KRT8 Antibody

• Catalog Number: orb1252102
• Category: Antibodies
• Description: KRT8 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CYKN8-1
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant human protein was used as the immunogen for this Cytokeratin 8 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 1-2 ug/mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT8
• UniProt ID: P05787
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8
• Note: For research use only
• Application notes: WB: 1-2 ug/mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of HeLa cell lysate with Cytokeratin 8 antibody. Expected molecular weight ~56kDa.

IHC testing of FFPE human lung carcinoma stained with Cytokeratin 8 antibody (CYKN8-1).