KRT19 Antibody Cocktail / Cytokeratin 19

• Catalog Number: orb385722
• Category: Antibodies
• Description: This mAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa + IgG2a, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: MCF-7 cells (A53-B/A2) and human mammary epithelial organoids (BA17) were used as the immunogen for this Cytokeratin 19 antibody cocktail.
• UniProt ID: P08727
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: A53-B/A2.26 + BA17
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cytokeratin 19 antibody cocktail is available for research use only.
• Note: For research use only

Western blot testing of human HepG2 cell lysate using Cytokeratin 19 antibody cocktail (clone A53-B/A2.26 + BA17). Predicted molecular weight ~43 kDa.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 19 antibody (A53-B/A2.26 + BA17).