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Catalog Number | orb639776 |
---|---|
Category | Antibodies |
Description | Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KRT15/2957 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2c, kappa |
Immunogen | Recombinant human KRT15 protein was used as the immunogen for the CK15 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Cytokeratin 15 antibody is available for research use only. |
UniProt ID | P19012 |
Storage | Store the Cytokeratin 15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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