KRT15 Antibody / Cytokeratin 15

• Catalog Number: orb639776
• Category: Antibodies
• Description: Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT15/2957
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: Recombinant human KRT15 protein was used as the immunogen for the CK15 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 15 antibody is available for research use only.
• UniProt ID: P19012
• Storage: Store the Cytokeratin 15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone KRT15/2957, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human MCF7 cells with Cytokeratin 15 antibody (clone KRT15/2957); Red=isotype control, Blue= Cytokeratin 15 antibody.

Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone KRT15/2957). Expected molecular weight ~50 kDa.

IHC staining of FFPE human colon carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human basal cell carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Cytokeratin 15 antibody (clone KRT15/2957). These results demonstrate the foremost specificity of the KRT15/2957 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 15 antibody (clone KRT15/2957) as confirmation of integrity and purity.