KRT14 Antibody

• Catalog Number: orb1252735
• Category: Antibodies
• Description: KRT14 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LL002
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG3
• Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT14
• UniProt ID: P02533
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Keratin, type I cytoskeletal 14, Cytokeratin-14, C
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human prostate (10X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of human prostate (20X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of FFPE human prostate with Cytokeratin 14 antibody.

IHC testing of FFPE human prostate with Cytokeratin 14 antibody.

Western blot testing of A431 cell lysate (epidermoid carcinoma) and Cytokeratin 14 antibody (clone LL002) at 1 ug/ml. Predicted/observed molecular weight: ~53kDa.