Cytokeratin 14/KRT14 Antibody

• Catalog Number: orb389423
• Category: Antibodies
• Description: Cytokeratin 14/KRT14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 14 (446-472aa RQIRTKVMDVHDGKVVSTHEQVLRTKN), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51561 MW
• UniProt ID: P02533
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Keratin, type I cytoskeletal 14;Cytokeratin-14;CK-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Cytokeratin 14 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-Cytokeratin 14 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cytokeratin 14 using anti-Cytokeratin 14 antibody.Lane 1:rat brain tissue;2:NIH3T3 cell;3:HEPG2 cell.

IHC analysis of Cytokeratin 14 using anti-Cytokeratin 14 antibody. Cytokeratin 14 was detected in paraffin-embedded section of human oesophagus squama cancer tissues.

IHC analysis of Cytokeratin 14 using anti-Cytokeratin 14 antibody. Cytokeratin 14 was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of Cytokeratin 14 using anti-Cytokeratin 14 antibody. Cytokeratin 14 was detected in paraffin-embedded section of human oesophagus squama cancer tissues.