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Catalog Number | orb1271011 |
---|---|
Category | Antibodies |
Description | KLRC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 26 kDa |
Target | KLRC1 |
UniProt ID | P26715 |
NCBI | P26715 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | NKG2-A/NKG2-B type II integral membrane protein, C Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of KLRC1 Antibody in Jurkat cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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