KIF4A antibody

• Catalog Number: orb18420
• Category: Antibodies
• Description: Goat polyclonal antibody to KIF4A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 1-2 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 140
• Target: KIF4A
• Protein Sequence: SFFSGCSPIEEEAH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KIF4A antibody, anti kinesin family member 4A
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysatesExperiment Notes: Immunofluorescence: This antibody has been successfully used in ICC/IF on HeLa (methanol-fixed, and BSA-blocked) by a customer.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HEK293 cells using KIF4A antibody

Western blot analysis of NIH3T3(lane 1) and Human Ovary(lane 2) lysate using KIF4A antibody

Immunofluorescence analysis of U2OS cells using KIF4A antibody