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Catalog Number | orb1523536 |
---|---|
Category | Antibodies |
Description | KAP-1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Mouse |
Immunogen | Between 175 and 225 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | KAP-1 |
UniProt ID | Q62318 |
NCBI | NP_035718.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | E3 SUMO-protein ligase TRIM28; Tif1beta; tripartit Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells, NIH 3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523536 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of mouse KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523536 used for IP at 6 µg/mg lysate.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
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