KAP-1 Antibody

• Catalog Number: orb1523535
• Category: Antibodies
• Description: KAP-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Mouse
• Immunogen: Between 175 and 225
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KAP-1
• UniProt ID: Q62318
• NCBI: NP_035718.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: E3 SUMO-protein ligase TRIM28; Tif1beta; tripartit
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells, NIH 3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523535 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523535 used for IP at 6 µg/mg lysate.