ISG15 Antibody

• Catalog Number: orb1270129
• Category: Antibodies
• Description: ISG15 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18 kDa
• Target: ISG15
• UniProt ID: P05161
• NCBI: P05161
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-like protein ISG15, Interferon-induced 1
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of ISG15 using rabbit polyclonal hISG15-A46 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2).

Western blot analysis in 293 cell line lysates (35 ug/lane).

Western blot analysis in Jurkat, K562 and AML-2 cell line lysates treated with a small molecular of 0, 10, 20, 30uM for 24 hours.