Integrin beta 4/ITGB4 Antibody

• Catalog Number: orb180705
• Category: Antibodies
• Description: Integrin beta 4/ITGB4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunofluorescence, 2μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 202167 MW
• UniProt ID: P16144
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Integrin beta-4;GP150;CD104;ITGB4;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-ITGB4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ITGB4 using anti-ITGB4 antibody.Lane 1:human A549 cell;2:human PC-3 cell;3:human T-47D cell;4:human HeLa cell;5:human Caco-2 cell.

IF analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human intestinal cancer tissues.