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Catalog Number | orb1152401 |
---|---|
Category | Antibodies |
Description | Integrin beta 4/ITGB4 Antibody (monoclonal, 7G10D2) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7G10D2 |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human |
Isotype | Mouse IgG2b |
Immunogen | E.coli-derived human Integrin beta 4/ITGB4 recombinant protein (Position: R29-K431). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 210 kDa |
UniProt ID | P16144 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Expiration Date | 12 months from date of receipt. |
IF analysis of ITGB4 using anti-ITGB4 antibody. ITGB4 was detected in an immunocytochemical section of A431 cells.
Western blot analysis of ITGB4 using anti-ITGB4 antibody.
Flow Cytometry analysis of MCF-7 cells using anti-ITGB4 antibody(Blue line).Isotype control antibody(Green line) was mouse IgG.Unlabelled sample(Red line) was also used as a control.
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