RACK1 Rabbit Polyclonal Antibody

• Catalog Number: orb6794
• Category: Antibodies
• Description: RACK1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RACK1 (221-317/317aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: RACK1
• UniProt ID: P63244
• RRID: AB_10927336
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cell proliferation-inducing gene 21 protein; GBLP_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4% Paraformaldehyde-fixed HepG2 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (RACK1) polyclonal Antibody, unconjugated (orb6794) 1:50, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RACK1) Polyclonal Antibody, Unconjugated (orb6794) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RACK1) Polyclonal Antibody, Unconjugated (orb6794) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.