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| Catalog Number | orb867326 |
|---|---|
| Category | Proteins |
| Description | Human LIF, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # P15018-1). |
| Target | LIF |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | P15018 |
| MW | 21.6 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Ser 23 - Phe 202 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

Immobilized Human LIF, His Tag (Cat. No. orb867326) at 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 1-20 ng/mL (QC tested).
> 95% by SDS-PAGE. | |
Recombinant Human LIF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser23-Phe202) of human LIF (Accession #NP_002300.1) fused with a 6��His Tag at the C-terminus. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human LIF R Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln45-Ser833) of Human LIF R fused with hFc His Tag tag at the C-terminal. |
>90%, determined by SDS-PAGE | |
This protein contains human LIFR (NP_001121143.1) (Met 1-Ser 833) with a C-terminal polyhistidine tag was expressed from HEK293 Cells. |
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