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Catalog Number | orb867326 |
---|---|
Category | Proteins |
Description | Human LIF, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # P15018-1). |
Target | LIF |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_002300.1 |
UniProt ID | P15018 |
MW | 21.6 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human LIF, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 1-20 ng/mL (QC tested). |
Expression Region | Ser 23 - Phe 202 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_002300.1 |
Immobilized Human LIF, His Tag (Cat. No. orb867326) at 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 1-20 ng/mL (QC tested).
> 90%, determined by SDS-PAGE | |
This protein contains human LIFR (NP_001121143.1) (Met 1-Ser 833) with a C-terminal polyhistidine tag was expressed from HEK293 Cells. |