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Mouse LIF Protein, His Tag
Catalog Number: orb334910
| Catalog Number | orb334910 |
|---|---|
| Category | Proteins |
| Description | Mouse LIF, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1). |
| Target | LIF |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Mouse |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_032527.1 |
| UniProt ID | P09056 |
| MW | 20.8 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.8 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Mouse |
| Expression Region | Ser 24 - Phe 203 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_032527.1 |
SDS-PAGE analysis of Mouse LIF protein
Mouse LIFR Protein, His Tag [orb2699214]
> 95%, determined by SDS-PAGE
This protein contains mouse LIFR (NP_0386121) (Met 1-Ser 828) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
100 μg, 50 μgMouse mLIFR Protein [orb752959]
Greater than 90.0% as determined by SDS-PAGE.
Sf9, Baculovirus cells
2 μg, 10 μg, 1 mgLIF Protein, Mouse, Recombinant (His) [orb1957219]
98.00%
21.3 kDa (predicted); 35-45 kDa (reducing condition, due to glycosylation)
100 μg, 1 mg, 20 μg
