CD4 Antibody

SKU: orb1261848

Description

CD4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1).
• Target: CD4
• Molecular Weight: Observed: 51kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

CD4 Antibody [9H5A8] : CD4mut

Western blot analysis of extracts of U-937 cells, using CD4 antibody at 1:500 dilution.

Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CD4

UniProt

P01730