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| Catalog Number | orb1261848 |
|---|---|
| Category | Antibodies |
| Description | CD4 Antibody |
| Target | CD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1). |
| UniProt ID | P01730 |
| MW | Observed: 51kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CD4 Antibody [9H5A8] : CD4mut |
| Research Area | Immunology & Inflammation |
| Note | For research use only |

Western blot analysis of extracts of U-937 cells, using CD4 antibody at 1:500 dilution.

Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Guinea pig, Rat, Sheep | |
Human, Mouse, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Monoclonal | |
Unconjugated |
Blocking, FC, IF, IHC-P, IP, NeA | |
Human, Primate | |
Mouse | |
Monoclonal | |
Unconjugated |
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