Importin 4 Antibody

• Catalog Number: orb1526111
• Category: Antibodies
• Description: Importin 4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 350 and 400
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-724A has not performed satisfactorily when used for WB of Importin 4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Importin 4.; IP - 5 - 15 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Importin 4
• UniProt ID: Q8TEX9
• NCBI: NP_078934.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: imp4b; importin-4; importin-4b; ran-binding protei
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Importin 4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Importin 4 antibody orb1526111 used for IP at 10 µg/mg lysate.

Detection of human Importin 4 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Importin 4 (Cat. No. orb1526111) used at a dilution of 1: 5, 000 (0.2µg/ml) Detection: DAB