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Catalog Number | orb18608 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to KPNA4 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:128000, WB: 0.5-1 μg/ml |
Conjugation | Unconjugated |
MW | 57.9 |
Target | KPNA4 / IPOA3 |
Entrez | 3840 |
Protein Sequence | NSSANVPTEGFQF |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti KPNA4 antibody, anti IPOA3 antibody, anti QIP Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concenWB: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1 μg/ml |
Expiration Date | 12 months from date of receipt. |
orb18608 staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18608 (3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18608 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of Human Testes (Lane 1), Human Ovary (Lane 2), Mouse Testes (Lane 3), Rat Testes (Lane 4) and Rat Ovary (Lane 5) lysate using KPNA4 antibody
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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