KPNA3 antibody

• Catalog Number: orb18610
• Category: Antibodies
• Description: Goat polyclonal antibody to KPNA3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 57.8
• Target: KPNA3 / IPOA4
• Entrez: 3839
• Protein Sequence: DPTANLQTKEFNF
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KPNA3 antibody, anti IPOA4 antibody, anti kar
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Heart shows strong pixulate staining of myocardial fibres in transverse section. Recommended concentration, 5-10ug/ml.WB: Approx 55-60kDa band observed in Human Testis and Kidney lysates (predicted size of approx. 58kDa according to NP_002258). Recommended for use at 0.1-0.5 μg/ml
• Expiration Date: 12 months from date of receipt.

orb18610 staining (0.1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18610 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of HeLa lysate using KPNA3 antibody

Immunohistochemical staining of Human heart using KPNA3 antibody