IL2RA Antibody

• Catalog Number: orb76260
• Category: Antibodies
• Description: IL2RA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha (39-57aa MAYKEGTMLNCECKRGFRR).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30819 MW
• UniProt ID: P01589
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-2 receptor subunit alpha;IL-2 receptor
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Anti-CD25/IL-2sR Alpha antibody, orb76260, Western blotting. All lanes: Anti CD25/IL-2sR Alpha (orb76260) at 0.5ug/ml. Lane 1: SMMC Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: JURKAT Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug, Lane 5: RAJI Whole Cell Lysate at 40ug, Lane 6: PANC Whole Cell Lysate at 40ug. Predicted bind size: 31KD. Observed bind size: 45KD.

Anti-CD25/IL-2sR Alpha antibody, orb76260, IHC (P) IHC (P): Human Tonsil Tissue.