Il2ra Antibody

• Catalog Number: orb1671485
• Category: Antibodies
• Description: Il2ra Antibody
• Clonality: Recombinant
• Clone Number: 2E4
• Tested applications: Depletion, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: The original version of this antibody was raised to recognize mouse CD25.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il2ra
• UniProt ID: P01590
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL-2-RA, Interleukin-2 receptor subunit alpha, IL-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD25 antibody 2E4 (Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 2E4 (orb1671485 - blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using Anti-CD25 antibody 2E4 (Mouse lymph node tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of 2E4 (orb1671485) at 0.5 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of mouse splenocytes with anti-CD25 (2E4 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric r version of 2E4 (orb1671485) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671485- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.