Il2ra Antibody

• Catalog Number: orb1671752
• Category: Antibodies
• Description: Il2ra Antibody
• Clonality: Recombinant
• Clone Number: PC-61.5.3
• Tested applications: Depletion, ELISA, FA, FC, IF, WB
• Reactivity: Mouse
• Isotype: IgG lambda
• Immunogen: This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il2ra
• UniProt ID: P01590
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL2-RA, IL-2 receptor subunit alpha,L-2R alpha cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of PC-61.5.3 (orb1671752) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671752- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.