IL2RA Antibody

• Catalog Number: orb1272673
• Category: Antibodies
• Description: IL2RA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SB-647
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot:To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: IL2RA
• UniProt ID: P01589
• NCBI: P01589
• Storage: sIL-2Ra lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Interleukin-2 receptor subunit alpha, TAC antigen,
• Note: For research use only
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot:To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions
• Expiration Date: 12 months from date of receipt.

This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.