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Catalog Number | orb157680 |
---|---|
Category | Antibodies |
Description | IL18 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Predicted Reactivity | Bovine, Equine, Porcine, Sheep |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human IL-18/IGIF (101-193/193aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 22 kDa |
Target | IL18 |
UniProt ID | Q14116 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Interleukin-18; interferon gamma-inducing factor I Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated (orb157680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated (orb157680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
WB | |
Human | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |