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Catalog Number | orb1958731 |
---|---|
Category | Proteins |
Description | IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231. |
Tag | Tag Free |
Purity | ≥ 95 % as determined by SDS-PAGE |
MW | 20.95 kDa (predicted); 19.7 kDa (reducing conditions) |
UniProt ID | P05231 |
Expression System | E. coli |
Biological Origin | Human |
Biological Activity | IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231. |
Expression Region | A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus. Predicted N terminal: Met |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide. The apparent molecular mass of mIL6-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 94% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result. |
> 90% as determined by SDS-PAGE method | |
20.8 kDa |