IL-18 Antibody

• Catalog Number: orb2635871
• Category: Antibodies
• Description: Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1a, IL-1b and IL-1g/IL-18 and a specific receptor antagonist, IL-1Ra. IL-1a and IL-1b play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1g) has been shown to augment the secretion of IFN-g from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1a and IL-1b respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1b pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-g-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IL18/4623
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant fragment (within amino acids 1-193) of human IL-18 protein was used as the immunogen for the Interleukin 18 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Interleukin 18 antibody is available for research use only.
• UniProt ID: Q14116
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Interleukin 18 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human A549 cell lysate with Interleukin 18 antibody. Predicted molecular weight ~22 kDa.

IHC staining of FFPE human adrenal gland tissue with Interleukin 18 antibody (clone IL18/4623). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Interleukin 18 antibody (clone IL18/4623). These results demonstrate the foremost specificity of the IL18/4623 mAb. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (clone MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free Interleukin 18 antibody (clone IL18/4623) as confirmation of integrity and purity.