IL-18 Antibody

• Catalog Number: orb1249615
• Category: Antibodies
• Description: IL-18 Antibody
• Target: IL18
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NPPDNIKDTKSDI
• UniProt ID: Q14116
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 23kDa band observed in lysates of cell line HeLa (calculated MW of 22.3kDa according to NP_001553.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL18, interleukin 18 (interferon-gamma-inducing fa
• Note: For research use only
• NCBI: NP_001553.1, NP_001230140.1

orb1249615 (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.