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Catalog Number | orb1958570 |
---|---|
Category | Proteins |
Description | IL-12B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.2 kDa and the accession number is P29460. |
Tag | C-His |
Purity | 97.10% |
MW | 36.2 kDa (predicted); 40-50 kDa (reducing condition, due to glycosylation) |
UniProt ID | P29460 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | IL-12B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.2 kDa and the accession number is P29460. |
Expression Region | A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ile 23 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation. | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
43 kDa |
> 95% by SDS-PAGE. | |
Recombinant Human IL-12B/P40 Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile23-Ser328) of human IL-12B/P40 (Accession #NP_002178.2) fused with a 6��His Tag at the C-terminus. |