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IL-12 Protein, Human, Recombinant (His)
Catalog Number: orb1957557
| Catalog Number | orb1957557 |
|---|---|
| Category | Proteins |
| Description | IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2. |
| Tag | C-His |
| Purity | 98.00% |
| UniProt ID | P29459 |
| MW | 60.13 kDa (predicted) |
| Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Expression System | HEK293 Cells |
| Biological Origin | Human |
| Biological Activity | IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2. |
| Expression Region | A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Ile 23 |
| Storage | -20°C |
| Note | For research use only |
- Item 1 of 3
Recombinant human IL-23 Alpha & mouse IL-12 Beta protein, C-His-Avi (HEK293) [orb1516411]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 50-60 (Mouse IL12 Beta) & 22 ( Human IL23 Alpha) kDa based on Tris-Bis PAGE result.
20 μg - Item 1 of 2
Recombinant human IL-12 protein, C-His-Avi (HEK293) [orb1516422]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 45/40 kDa based on Tris-Bis PAGE result.
50 μg - Item 1 of 1
Human IL12B Protein, His Tag [orb1290821]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 1
Recombinant human IL-12B protein, C-His (HEK293) [orb1817206]
> 90% as determined by SDS-PAGE
43 kDa
100 μg, 500 μg Recombinant Human IL12RB2 Protein, N-His [orb2834948]
> 90% as determined by SDS-PAGE.
17.55 kDa
100 μg, 50 μg, 20 μg
