IKBKB Antibody

• Catalog Number: orb1239687
• Category: Antibodies
• Description: IKBKB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.The immunogen is located within amino acids 650 - 700 of IKK beta.
• Concentration: 1 mg/ml
• Dilution range: IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 87 kDa
• Target: IKBKB
• UniProt ID: O14920
• NCBI: O14920
• Storage: IKK beta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IKK beta Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IKK beta Antibody: IKK2, IKKB, IMD15, NFKBIKB, IKK
• Note: For research use only
• Application notes: IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 µg/mL.

Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 µg/mL.