IKK beta/IKBKB Antibody

• Catalog Number: orb215975
• Category: Antibodies
• Description: IKK beta/IKBKB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 86564 MW
• UniProt ID: O14920
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Inhibitor of nuclear factor kappa-B kinase subunit
• Note: For research use only
• Application notes: WB: The detection limit for IKK beta is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Human Lung Cancer Tissue using Anti-IKK beta antibody.

Flow Cytometry analysis of 293T cells using anti-IKK beta antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using Anti-IKK beta antibody.Lane 1: HEPG2 Cell;2:COLO320 Cell;3: M231 Cell;4: HT1080 Cell.

WB analysis of Recombinant Human IKK beta Protein 0.5ng using Anti-IKK beta antibody.

IF analysis of IKK beta using anti-IKK beta antibody. IKK beta was detected in immunocytochemical section of A431 cells.