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IgM Heavy Chain Antibody

Catalog Number: orb639730

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb639730
CategoryAntibodies
DescriptionRecognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
ClonalityRecombinant
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenHeavy chain of human IgM was used as the immunogen for the recombinant IgM Heavy Chain antibody.
UniProt IDP01871
Tested applicationsFACS, IF, IHC-P
Dilution rangeFlow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application notesOptimal dilution of the antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberrIGHM/2558
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant IgM Heavy Chain antibody is available for research use only.
NoteFor research use only
IgM Heavy Chain Antibody

IHC staining of FFPE human tonsil with recombinant IgM Heavy Chain antibody (clone rIGHM/2558). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IgM Heavy Chain Antibody

IHC staining of FFPE human tonsil with recombinant IgM Heavy Chain antibody (clone rIGHM/2558). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IgM Heavy Chain Antibody

SDS-PAGE analysis of purified, BSA-free recombinant IgM Heavy Chain antibody (clone rIGHM/2558) as confirmation of integrity and purity.

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