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IgM Heavy Chain Antibody

Catalog Number: orb2637755

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2637755
CategoryAntibodies
DescriptionThis antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2b, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenRecombinant heavy chain of human IgM was used as the immunogen for this antibody.
Tested applicationsFACS, IF, IHC-P, WB
Dilution rangeFlow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberIM373
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis IgM heavy chain antibody is available for research use only.
NoteFor research use only
Entrez3507
IgM Heavy Chain Antibody

IHC testing of human tonsil stained with IgM heavy chain antibody.

IgM Heavy Chain Antibody

IHC testing of human tonsil stained with IgM heavy chain antibody.

IgM Heavy Chain Antibody

Western blot testing of Raji cell lysate with IgM heavy chain antibody. Expected molecular weight: 70-75 kDa.

IgM Heavy Chain Antibody

SDS-PAGE Analysis of Purified, BSA-Free IgM Heavy Chain Antibody (clone IM373). Confirmation of Integrity and Purity of the Antibody.

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