IDE Antibody

• Catalog Number: orb1268222
• Category: Antibodies
• Description: IDE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 118 kDa
• Target: IDE
• UniProt ID: P14735
• NCBI: P14735
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Insulin-degrading enzyme, Abeta-degrading protease
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-IDE Antibody in A375 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of lysate from K562 cell line, using IDE Antibody at 1:1000.