IDE Antibody

• Catalog Number: orb1247359
• Category: Antibodies
• Description: IDE Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QQYNFDRDNTE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IDE
• UniProt ID: P14735
• NCBI: NP_001159418.1, NP_001309726.1, NP_001309723.1, NP_001309724.1, NP_001309722.1, NP_004960.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: insulinase, insulysin, insulin-degrading enzyme ,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247359 (0.1 ug/ml) staining of K562 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247359 (2.5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247359 (2.5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.