Insulin degrading enzyme/IDE Antibody

• Catalog Number: orb413045
• Category: Antibodies
• Description: Insulin degrading enzyme/IDE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human IDE recombinant protein (Position: F485-K756).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 118 kDa
• UniProt ID: P14735
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Insulin-degrading enzyme; Abeta-degrading protease
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human Hela whole cell lysates(Lane 1)

human placenta tissue lysates(Lane 2), human COLO-320 whole cell lysates(Lane 3), human HepG2 whole cell lysates(Lane 4), human SGC-7901 whole cell lysates(Lane 5), human Jurkat whole cell lys

IHC analysis of human mammary cancer tissue using IDE antibody

IHC analysis of rat pancreas tissue using IDE antibody

Flow Cytometry analysis of Hela cells using IDE antibody