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Human VEGF165 Protein
Catalog Number: orb257923
| Catalog Number | orb257923 |
|---|---|
| Category | Proteins |
| Description | Human VEGF165, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
| Target | VEGF165 |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_001165097.1 |
| UniProt ID | P15692 |
| MW | 19.2 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 19.2 kDa. The protein migrates as 24 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.01 EU per μg |
| Biological Origin | Human |
| Biological Activity | Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. ) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Ala 27 - Arg 191 |
| Storage | -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_001165097.1 |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human VEGF protein
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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- Item 1 of 1
Human VEGF165 Protein, hFc Tag [orb2321384]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg 
Human VEGF165 Protein [orb1289549]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μg- Item 1 of 1
Recombinant human VEGF-165 protein (Active, HEK293) [orb1817182]
> 95% as determined by SDS-PAGE
39-45 kDa
50 μg, 10 μg - Item 1 of 1
Recombinant human VEGF165 protein, C-His [orb1516905]
> 95% as determined by SDS-PAGE
20 kDa
500 μg, 100 μg
