Human VEGF165 Protein

• Catalog Number: orb257923
• Category: Proteins
• Description: VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 19.2 kDa
• Target: VEGF165
• Source: Human VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
• Expression System: HEK293
• Biological Activity: Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ala 27 - Arg 191
• Endotoxins: 0.01 EU per μg
• NCBI: NP_001165097.1
• Storage: -20°C to -70°C for 24 months in lyophilized state; -70°C for 24 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).