Human VEGF165 Protein

SKU: orb257923

Description

Human VEGF165, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).

Images & Validation

• Application Notes:
  This protein carries no "tag". The protein has a calculated MW of 19.2 kDa. The protein migrates as 24 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: VEGF165
• Reactivity: Human
• Tag: Native
• Molecular Weight: 19.2 kDa
• Expression Region: Ala 27 - Arg 191
• Purity: 95%
• Endotoxins: 0.01 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Disclaimer: For research use only

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Quick Database Links

Gene Symbol

VEGF165

UniProt

P15692