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Description
Research Area
Cardiovascular Research
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human VEGF-A Antibody (Bevacizumab) (Cat#orb2992936) on Pro-A Biosensor, can bind Recombinant Human VEGF165 (N-6His) (Cat#orb2992805) with an affinity constant of 2.11 pM as determined in BLI assay. (Regularly tested) |
| Tag | N-6His |
| Expression Region | Ala27-Arg191 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 20 KDa. Observed: 18-28 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Vascular Endothelial Growth Factor Isoform 165; VEGF165
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide