Human TNF-alpha Protein

• Catalog Number: orb383503
• Category: Proteins
• Description: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 18.3 kDa
• Target: TNF-alpha
• Source: Human TNF-alpha, His Tag (active trimer) (MALS verified) (orb383503) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).
• Expression System: HEK293
• Biological Activity: Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Val 77 - Leu 233
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000585.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TNF-alpha protein

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. orb383503) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).