Human TNF-alpha Protein

SKU: orb257883

Description

Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).

Research Area

Cytokines

Images & Validation

• Tested Applications: ELISA, SDS-PAGE
• Application Notes:
  This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: TNF-alpha
• Reactivity: Human
• Tag: Native
• Molecular Weight: 17.4 kDa
• Expression Region: Val 77 - Leu 233
• Purity: >95% as determined by SDS-PAGE and SEC-MALS.
• Endotoxins: Less than 0.01 EU per μg by the LAL method / rFC method.
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Disclaimer: For research use only

Alternative Names

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

SDS-PAGE analysis of Human TNF-alpha protein

Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Quick Database Links

Gene Symbol

TNF-alpha

UniProt

P01375