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Human TNF-alpha Protein

Catalog Number: orb257883

DispatchUsually dispatched within 5-10 working days
$ 220.00
Catalog Numberorb257883
CategoryProteins
DescriptionHuman TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
ReactivityHuman
TagNative
Form/AppearancePowder
Purity95%
ConjugationUnconjugated
MW17.4 kDa
TargetTNF-alpha
UniProt IDP01375
SourceHuman TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
Expression SystemHEK293
Biological OriginHuman
Biological ActivityHumira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Expression RegionVal 77 - Leu 233
Endotoxins0.01 EU per μg
NCBINP_000585.2
Storage-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesDIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
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NoteFor research use only
Application notesThis protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Human TNF-alpha Protein

SDS-PAGE analysis of Human TNF-alpha protein

Human TNF-alpha Protein

Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations

Human TNF-alpha Protein

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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