Human TNF-alpha Protein

• Catalog Number: orb257883
• Category: Proteins
• Description: Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
• Target: TNF-alpha
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_000585.2
• UniProt ID: P01375
• MW: 17.4 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.01 EU per μg
• Biological Origin: Human
• Biological Activity: Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. ) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Val 77 - Leu 233
• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000585.2
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TNF-alpha protein

Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).