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Catalog Number | orb257883 |
---|---|
Category | Proteins |
Description | Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 17.4 kDa |
Target | TNF-alpha |
UniProt ID | P01375 |
Source | Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Val 77 - Leu 233 |
Endotoxins | 0.01 EU per μg |
NCBI | NP_000585.2 |
Storage | -20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TNF-alpha protein
Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Greater than 90% as determined by SDS-PAGE. | |
19.4 kDa | |
Yeast |
FACS, IF, IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |